Part 1: Benchling & In-silico Gel Art

*See the [Gel Art: Restriction Digests and Gel Electrophoresis](<https://docs.google.com/document/d/1TTZiQ79mN4w9euS2bycKIc9f0U0BC7MuvJ7_hW_7qcg/edit#heading=h.jyt74412izch>) protocol for details. Overview:*

• [ ] Make a free account at [benchling.com](<http://benchling.com>)
• [ ] Import the [Lambda DNA](<https://www.neb.com/products/n3011-lambda-dna#Product%20Information>).
• [ ] Simulate Restriction Enzyme Digestion with the following Enzymes: *EcoRI HindIII BamHI KpnI EcoRV SacI SalI*
• [ ] Create a pattern/image in the style of Paul Vanouse’s [Latent Figure Protocol](<https://www.paulvanouse.com/lfp.html>) artworks.

First, I went to https://www.neb.com/en-us/tools-and-resources/interactive-tools/dna-sequences-and-maps-tool and downloaded the FASTA format sequence file, and uploaded it to benchling under New project > DNA and RNA sequence > Upload.

Next, I use the “digest” (scissors) tool and entered the various restriction enzymes to cut the sequence.

Restriction enzymes

• EcoRI-HF
• HindIII-HF
• BamHI-HF
• KpnI-HF
• EcoRV-HF
• SacI-HF
• SalI-HF